Isabelle Migeotte

http://migeotte.ulb.be/wordpress/

Our research studies the cellular, molecular, and mechanical mechanisms of coordinated migration, in which cells are physically and/or functionally connected, using the early mouse embryo as a model.

Collective migration is essential for morphogenetic movements during embryogenesis, as well as for major physiological (such as wound healing) and pathological (such as cancer invasion) processes during adult life. The peri-gastrulation mouse embryo is a relatively simple structure composed of a limited number of distinguishable cell types, wich allows studying specific cell populations in their physiological context.

In particular, we focus on  Anterior Visceral Endoderm migration as a model of epithelial collective migration, and Mesoderm migration as a model of epithelial-mesenchymal transition followed by cell sheet migration. We use quantitative live imaging and transcriptomics to decipher the determinants of early morphogenesis, the ultimate goal being to reproduce in vitro the extracellular milieu that will drive endoderm and mesoderm cells towards a particular fate.

Nahaboo and Migeotte, eLS 2018)

Isabelle Migeotte – Principal Investigator  Research Associate F.N.R.S (imigeott@ulb.ac.be)

phone # +32 (0)2 555 3017

Isabelle Migeotte graduated as a Medical Doctor (1998), received her PhD in Medical Sciences (2005) and was certified in internal medicine in 2005, specialised in medical genetics, at the Université Libre de Bruxelles (ULB), Belgium. Isabelle performed a postdoctoral training in Kathryn Anderson’s laboratory, Memorial Sloan-Kettering Cancer Center, NY, USA (2005-2010).

Isabelle is a FNRS Research Associate, IRIBHM, ULB and a Consultant in Medical Genetics, Hôpital Erasme, ULB

Wallis Nahaboo, PhD – postdoctoral fellow

After completing her biomedical engineer diploma (PHELMA, Grenoble INP), Wallis started her Phd in Delattre lab in ENS Lyon, France.She worked on the forces that drive chromosome segregation in C. elegans embryos. Wallis is interested in studying live cell dynamics and specifically the cytoskeleton.Just after her PhD defence, in April 2016, she joined our lab as a postdoc. She is currently working on cell migration in mouse embryos development: She focuses on quantitative analysis of imaging data, as well as develops tools to study mechanical forces in the gastrulating embryo.

Evangéline Despin-Guitard, PhD Student

Selected Publications

Navrita Mathiah*, Evangéline Despin-Guitard*, Matthew Stower, Wallis Nahaboo, Elif Sema Eski, Sumeet Pal Singh, Shankar Srinivas, Isabelle Migeotte, An asymmetry in the frequency and position of mitosis in the epiblast precedes gastrulation and suggests a role for mitotic rounding in cell delamination during primitive streak epithelial-mesenchymal transition;
Biorxiv doi: https://doi.org/10.1101/2020.02.21.959080

Nahaboo, Wallis, and Migeotte, Isabelle(2018) Cleavage and Gastrulation in the Mouse Embryo. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0001068.pub3]

 Browet, A. A., De Vleeschouwer, C., Jacques, L. L., Mathiah, N., Saykali, B., & Migeotte, I. (2016). Cell segmentation with random ferns and graph-cuts. Proceedings – International Conference on Image Processing, 7533140, 4145-4149. doi:10.1109/ICIP.2016.7533140

Mazari, E., Zhao, X., Migeotte, I., Collignon, J., Gosse, C., & Perea-Gomez, A. (2014). A microdevice to locally electroporate embryos with high efficiency and reduced cell damage. Development, dev.106633. http://doi.org/10.1242/dev.106633

Zhao, X., Mazari, E., Suárez-Boomgaard, D. D., Migeotte, I., Perea-Gomez, A., & Gosse, C. (2014). Finite element model simulations to assist the design of microdevices dedicated to the localized electroporation of mouse embryos. E C S Transactions, 64(16), 7 14. doi:10.1149/06416.0007ecst

Simonis, N., Migeotte, I., Lambert, N., Perazzolo, C., de Silva, D. C., Dimitrov, B., Heinrichs, C., Janssens, S., Kerr, B., Mortier, G., Van Vliet, G., Lepage, P., Casimir, G., Abramowicz, M., Smits, G., & Vilain, C. (2013, June). FGFR1 mutations cause Hartsfield syndrome, the unique association of holoprosencephaly and ectrodactyly. Journal of medical genetics, 50(9), 585-592. doi:10.1136/jmedgenet-2013-101603

Bloomekatz, J., Grego-Bessa, J., Migeotte, I., & Anderson, K. V. (2012). Pten regulates collective cell migration during specification of the anterior-posterior axis of the mouse embryo. Developmental Biology, 364(2), 192–201. http://doi.org/10.1016/j.ydbio.2012.02.005

Migeotte, I., Grego-Bessa, J., & Anderson, K. V. (2011). Rac1 mediates morphogenetic responses to intercellular signals in the gastrulating mouse embryo. Development (Cambridge, England), 138(14), 3011–3020. http://doi.org/10.1242/dev.059766

Lee, J. D., Migeotte, I., & Anderson, K. V. (2010). Left-right patterning in the mouse requires Epb4.1l5-dependent morphogenesis of the node and midline. Developmental Biology, 346(2), 237–246. http://doi.org/10.1016/j.ydbio.2010.07.029

Migeotte, I., Omelchenko, T., Hall, A., & Anderson, K. V. (2010). Rac1-dependent collective cell migration is required for specification of the anterior-posterior body axis of the mouse. PLoS Biology, 8(8), 37–38. http://doi.org/10.1371/journal.pbio.1000442