Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire

Transgenesis

[ C.Ledent , B.Robaye, P.Vanderhaeghen]

The IRIBHM has developed a mouse transgenic facility in the latest eighties. Addition transgenesis, by microinjection of DNA constructs into fertilized mouse eggs, and gene replacement, resulting from the genetic manipulation of embryonic stem cells (ES), are available in the institute. These techniques have been used to generate models of thyroid diseases and to study the role in vivo of G protein coupled receptors, signalling proteins and factors involved in  brain development.

 

Main recent publications

Depaepe V, Suarez N, Passante L, Dufour A, Gorski J, Jones K, Ledent C, and Vanderhaeghen P.

Ephrin signalling controls brain size by regulating apoptosis of neural progenitors.
Nature 435: , 1244-1250 (2005)

Ledent C, Demeestere I, Blum D, Petermans J, Hamalainen T, Smits G, Vassart G.

Premature ovarian aging in mice deficient for Gpr3.
Proc.Natl.Acad.Sci.U.S A 102:8922-6 (2005).

Laurent P, Becker JA, Valverde O, Ledent C, de Kerchove d'Exaerde A, Schiffmann SN,
Maldonado R, Vassart G, Parmentier M.

The prolactin-releasing peptide antagonizes the opioid system through its receptor GPR10.
Nat Neurosci. 8(12):1735-4 (2005).

Jardinaud F, Crete D, Canestrelli C, Ledent C, Roques BP, Noble F.

CB1 receptor knockout mice show similar behavioral modifications to wild-type mice when
enkephalin catabolism is inhibited.
Brain Res. 1063(1):77-83 (2005).

Ledent C, Demeestere I, Blum D, Petermans J, Hamalainen T, Smits G, Vassart G.

Premature ovarian aging in mice deficient for Gpr3.
Proc Natl Acad Sci U S A. 102(25):8922-6 (2005).

Laurent P, Becker JA, Valverde O, Ledent C, de Kerchove d'Exaerde A, Schiffmann SN,
Maldonado R, Vassart G, Parmentier M.

The prolactin-releasing peptide antagonizes the opioid system through its receptor GPR10.
Nat Neurosci. 8(12):1735-41 (2005).

Ghanem E, Lovdahl C, Dare E, Ledent C, Fredholm BB, Boeynaems JM, Van Driessche W,
Beauwens R.

Luminal adenosine stimulates chloride secretion through A1 receptor in mouse jejunum.  
Am J Physiol Gastrointest Liver Physiol. 288(5):G972-7 (2005).

Mievis S, Levivier M, Vassart G, Brotchi J, Ledent C, Blum D.

Citicoline is not protective in experimental models of Huntington's disease.
Neurobiol Aging. 2006

Castane A, Soria G, Ledent C, Maldonado R, Valverde O.

Attenuation of nicotine-induced rewarding effects in A2A knockout mice.
Neuropharmacology.51(3):631-40 (2006).

Mikics E, Dombi T, Barsvari B, Varga B, Ledent C, Freund TF, Haller J.

The effects of cannabinoids on contextual conditioned fear in CB1 knockout and CD1 mice.
Behav Pharmacol. 17(3):223-30   2006.

Short JL, Ledent C, Borrelli E, Drago J, Lawrence AJ.

Genetic interdependence of adenosine and dopamine receptors: evidence from receptor
knockout mice.
Neuroscience. 139(2):661-70 (2006).

Castane A, Celerier E, Martin M, Ledent C, Parmentier M, Maldonado R, Valverde O.

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