Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire

Two hybrid

[ I.Pirson ]

Contribution to the elucidation of a protein's function can benefit greatly from the identification of its protein partners. The two-hybrid in vivo method of screening for protein/protein interactions in yeast, in now enabling research teams to identify potential protein interactors of a variety of proteins of interest. Our lab's main research thrust is focused on protein/protein interactions in the field of signal transduction. We have developed diverse biochemical and cellular biology methods to support the results yielded by the two-hybrid system and to address the physiological relevance of the identified complexes.


Main recent publications

Mircescu*H., Steuve*S., Savonet V., Degraef C., Mellor H., Dumont J.E., Maenhaut C., Pirson I.

Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain and whose expression is specifically modulated in thyroid cells by cAMP.
European Journal of Biochemistry 269: 6241-6249 (2002).

Vandenbroere I., Paternotte N., Dumont J.E., Erneux C., Pirson I.

The c-Cbl associated protein (CAP) and c-Cbl are two new partners of the SH2-containing inositol polyphosphate 5- phosphatase SHIP2.
Biochemical and Biophyical Communications 300 : 494-500 (2003).

Arsenijevic T., Degraef C., Dumont J.E., Roger P.P.and Pirson I.

A novel partner for cyclins D: Protein Kinase A-anchoring protein AKAP95.
Biochemical Journal 378: 673-679 (2003).

Jacobs C., Onnockx S, Vandenbroere I. and Pirson I.

Endogenous SHIP2 does not localize in lipid rafts in 3T3-L1 adipocytes.
FEBS Letters 565: 70-74 (2004).

Paternotte N., Zhang J., Vandenbroere I., Backers K., Blero D., Kioka N., Vanderwinden J-M, Pirson I., and Erneux C.

SHIP2 interaction with the cytoskeletal protein Vinexin.
FEBS Journal  272: 6052-6066 (2005)

Arsenijevic T, Degraef C, Dumont JE, Roger PP, Pirson I.

G1/S Cyclins interact with regulatory subunit of PKA via A-kinase anchoring protein, AKAP95.
Cell Cycle. 11 :1217-22 (2006).

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