Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire

Metabolism of phosphoinositides

[ David Communi, C.Erneux, I.Pirson ]

Since 1985, our work at the Institute has consisted in the definition at the molecular level of the mechanisms and enzymes involved in the signal termination reaction of new important intracellular molecules. The defined mechanisms, the cloning of new enzymes such as InsP3 3-kinases, 5-phosphatases, SHIP1 and SHIP2 have introduced new concepts and new targets in pharmacology. Loss of SHIP2 in mice leads to increased sensitivity to insulin.


Main recent publications

Daniel Blero, Bernard Payrastre, Stéphane Schurmans, Christophe Erneux.

Phosphoinositide phosphatases in a network of signalling reactions
Pflugers Arch - Eur J Physiol (2007) 455:31–44

Giuriato S., Pesesse X., Bodin S., Sasaki T., Viala C., Marion E., Penninger J., Schurmans S., Erneux C., and Payrastre B.

SH2 domain containing inositol 5-phosphatases 1 and 2 in blood platelets: interaction and respective role in the control of phosphatidylinositol 3,4,5-trisphosphate level.
Biochem J., 376:199-207 (2003).

Pouillon V., Hascakova-Bartova R., Pajak B., Adam E., Bex F., Dewaste V., Van Lint C., Leo O., Erneux C., and Schurmans S.

Inositol 1,3,4,5-tetrakisphosphate is essential for normal T lymphocyte development.
Nature Immunol., 4:1136-1143 (2003).

Schurmans S.

Ship2 and insulin sensitivity: a role in type 2 diabetes?
Bull. Mem. Acad. R. Med. Belg., 158: 335-9 (2003).

Kaisaki P.J., Delépine M., Woon P. Y., Sebag-Montefiore L., Wilder S.P., Menzel S., Vionnet N., Marion E., Riveline J.P., Charpentier G., Schurmans S., Levy J.C., Lathrop M., Farrall M., and Gauguier D.

Polymorphisms in Type-II SH2 domain-containing Inositol 5-Phosphatase (INPPL1, SHIP2) are Associated with Physiological Abnormalities of the Metabolic Syndrome.
Diabetes 53: 1900-1904 (2003).

Hascakova-Bartova R., Pouillon V., Dewaste V., Moreau C., Jacques C., Banting G., Schurmans S., and Erneux C.

Identification and subcellular distribution of endogenous Ins(1,4,5)P3 3-kinase B in mouse tissues.
Biochem. Biophys. Res. Commun., 323: 920-925 (2003).

Wang Y., Keogh R. J., Hunter M. G., Mitchell C. A., Frey R. S., Javaid K., Malik A. B., Schurmans S., Tridandapani S., and Marsh C. B.

SHIP2 is recruited to the cell membrane upon M-CSF stimulation and regulates M-CSF-induced signaling.
J. Immunol. 173: 6820-6830 (2003).

Jacobs C., Onnockx S, Vandenbroere I. and Pirson I.

Endogenous SHIP2 does not localize in lipid rafts in 3T3-L1 adipocytes.
FEBS Letters 565: 70-74 (2004).

Paternotte N., Zhang J., Vandenbroere I., Backers K., Blero D., Kioka N., Vanderwinden J-M, Pirson I., and Erneux C.

SHIP2 interaction with the cytoskeletal protein Vinexin.
FEBS Journal  272: 6052-6066 (2005).

Blero D., Zhang J., Pesesse X., Payrastre B., Dumont J.E., Schurmans S., and Erneux C.

Phosphatidylinositol 3,4,5-trisphosphate modulation in Ship2-deficient mouse embryonic fibroblasts.
FEBS J., 272: 2512-2522 (2005).

Poinas, A., Backers, K., Riley, A., Mille, S., Moreau, C., Potter, B.V.L. and Erneux, C.

I nteraction of the catalytic domain of Ins(1,4,5)P3 3-kinase A with inositol phosphate analogues.
BioChem Biophys Res   6:, 1449-1457 (2005).

Harada, K., Takeuchi, H., Oike, M., Matsuda, M., Katenatsu, T., Yagisawa, H., Nakayama, K.I., Maeda, K., Erneux, C. and Hirata, M.

Role of PRIP-1, a novel Ins(1,4,5)P3 binding protein in Ins(1,4,5)P3 ­ mediated Ca2+ signaling
J Cell Physiol   202: 422-433 (2005).

Markadieu, N, Crutzen, R, Blero, D., Erneux, C, Beauwens, R.

Hydrogen peroxide and epidermal growth factor activate phosphatidylinositol 3-kinase and increase sodium transport in A6 cell monolayers
Am J. Renal Physiol   288(6):F1201-12 (2005).

Vanderwinden, J.M., Wang, D., Paternotte, N., Mignon, S., Isozaki, K. and Erneux, C.

Differences in activated signaling pathways and in expression level of the phosphoinositides phosphatase SHIP1 between two oncogenic mutants of the receptor tyrosine kinase KIT
Cellular Signalling 18: 661-669 (2006).

Van Sande, J., Dequanter, D., Massart, C., Lothaire, P., Dumont, J. E. and Erneux, C.

TSH stimulates the generation of inositol 1,4,5-trisphosphate in human thyroid cells
J Clin Endocrinology & Metabolism   91: 1099-1107 (2006).

Vandeput, F., Backers, K., Villeret, V., Pesesse, X., Erneux, C.

The influence of anionic lipids on SHIP2 phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase activity
Cellular Signalling 18(12):2193-2199 (2006) .

Gloire, G., Charlier, E., Rhamouni, S., Volanti, C., Chariot, A., Erneux, C., Piette, J.

Restauration of SHIP-1 activity in human leukemic cells modify NF- B activation pathway and cellular survival upon oxidative stress treatment
Oncogene 25(40):5485-4594 (2006).

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